ABSTRACT
Objective:To compare the effectiveness between three methods for purifying the immunoglobulin of egg yolk(IgY) which are polyethylene glycol (PEG) method, chloroform extraction method and chloroform / PEG method, and to provide basis for obtaining the batch of IgY.Methods:The inactivated vaccine of Vibrio parahemolyticus (V. parahemolyticus) was prepared and the hens were immunized by multi-point intramuscular injection.The eggs were collected and the IgY was purified by PEG method, chloroform extraction method and chloroform/PEG method.The protein extraction rate, the IgY titer and the purity of the antibody which purified by different methods were detected.Furthermore, the operation process, cost and safety of the three methods were analyzed.Results:The protein contents of the extraction belonging three methods from high to low in turn were chloroform extraction method, chloroform/PEG method, and PEG method.There was no significant difference in the antibody titer between three methods, and the tiler of chloroform extraction method was slightly high.The purities of purified antibody from high to low in turn were PEG method, chloroform/PEG method and chloroform method.The PEG method had better security but relatively lower extraction efficiency and higher cost.The chloroform/PEG method had high extraction efficiency and good antibody purity.Conclusion:The PEG method is suitable for a small amount of extraction in the laboratory.The chloroform/PEG method is appropriate for extracting the high quality IgY in a batch as it has high extraction efficiency and good antibody purity.
ABSTRACT
Objective To analyze the MSCT manifestations of septic pulmonary embolism(SPE)to explore the diagnosis value of MSCT.Methods The MSCT findings of eight patients with SPE were analyzed retrospectively.Results ① Of all 177 lesions,84 lesions located in both upper lobes,25 lesions located in right middle lobe and 68 lesions located in both lower lobes.② There were 106 nodules and 44 wedge shaped opacities in all the 8 cases,of which the 88 nodules (83.0%)and all of the wedge shaped opacities located in sub-pleural zones of the lung and the 18 nodules (1 7.0%)located in the inner and middle lung fields.In 6 cases,the 26 nodules (24.5%)and the 1 6 wedge shaped lesions (38.1%)had “feeding vessels”.③ There were 27 patchy shadows in 6 patients,of which 12 patchy shadows (44.4%)had air bronchogram.④ There were 18 cavities in 6 patients,of which 14 cavities (13.2%)located in nodules and 4 cavities (9.1%) in the wedge shaped lesions.There were 8 cysts in 4 patients,of which 6 cysts located in nodules (5.7%)and 2 cysts in the wedge shaped lesions (4.5%).⑤ The nodules and the wedge shaped lesions were enhanced slightly.⑥ Pleural effusion and pericardial effusion were found in 4 and 3 patients,repectively.⑦ Of 6 patients the follow-up MSCT scan in a short period showed the disease progressions. Conclusion SPE has characteristic manifestations in MSCT,and MSCT can provide valuable information for the diagnosis of SPE.